The E. coli strain ER2738 is an amber suppressor (glnV) F⁺Êstrain with a rapid growth rate and is particularly well-suited for M13 phage propagation. Amid BiosciencesÕ high efficiency electrocompetent ER2738 cellsÊcan be used forÊphage display,Êantibody phage display, library constructionÊand screening. The F-factor of ER2738 contains a mini-transposon which confers tetracycline resistance, so cells can be selected by plating and propagating in tetracycline-containing medium. ER2738 cultures for infection can be grown either in LB or LB+Tet media. Tetracycline does not need to be added to media during phage amplification. Loss of F-factor in nonselective media is insignificant as long as cultures are not serially diluted. Although ER2738 is a recA+ strain, there are no reports on spontaneous in vivo recombination events with M13 or phagemid vectors.

For Research Use Only (RUO). This product is for research use only. It is not intended for diagnostic or therapeutic use in humans or animals.